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A. Nomura, J. Yamada, T. Yarita, Y. Sudo, S. Kudo, and Y. Nishizawa
[Anal. Sci., Vol.11, pp.385-388, 1995]
Free Fatty Acids (FFAs) were determined by supercritical fluid chromatography (SFC) on an inert ODS silica-gel column using carbon dioxide as a mobile phase without a modifier. The standard samples of FFAs with carbon numbers of 6 to 26 (even numbers) were completely separated using the pressure-programming mode. The retention behavior of FFAs was investigated in terms of the density of the mobile phase, and the FFAs were separated according to a reversed-phase mode in liquid-chromatographic terms. The composition of fatty acids of coconut oil was determined by the SFC, and was compared with gas chromatography. A good agreement was obtained. The chromatographic separation of FFAs obtained from fish oil as well as the enzymatic hydrolysis products of the fish oil were also conducted.