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The present authors have developed the method for direct observation of aluminum in bone tissues by a real time laser scanning confocal microscope after staining aluminum with Lumogallion, the most sensitive fluorescent reagents for aluminum. Polished tibia tissue specimens were examined with Lumogallion stain for aluminum using laser scanning confocal microscope. Tibia preparations showed an intense fluorescent coloration on the medullary aspect, indicating accumulation of aluminum. An abnormal accumulation of aluminum in the tibia was demonstrated following administration of an aluminum preparation in rats with chronic renal failure.